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Alu Human DNA Typing Using PCR
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.
*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.
| Cat. No.: | 333 |
| Group Size: | For 25 students |
| Time Required: | Extraction – 50 minutes
PCR Setup – 10 minutes PCR – 90 minutes Electrophoresis – 60 minutes Staining – 5 minutes to overnight |
| Kit Includes: | Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube. |
| All You Need: | DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate. |
| Storage: | Some Components Require Freezer Storage Upon Receipt |
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