BeyoCRISPRTM Quick Construction Kit (mOrange2 Reporter)

Beyotime’s BeyoCRISPR Quick Construction Kit (mOrange2 Reporter) allows rapid plasmid construction for expression of Cas9 and specific guide RNAs (gRNAs) in mammalian cells. Users only need to synthesize target-specific DNA oligos, anneal to form double strands, and then ligate to the linearized vector provided in this kit for CRISPR/Cas9-based gene editing of interest. After transfection, positive mammalian cells expressing Cas9 and gRNA can be screened by orange-red fluorescent mOrange2, where gene editing usually occurs.