BeyoCRISPRTM Quick Construction Kit (Puro)

Beyotime’s BeyoCRISPR Quick Construction Kit (Puro) allows rapid plasmid construction for expression of Cas9 and specific guide RNAs (gRNAs) in mammalian cells. Users only need to synthesize target-specific DNA oligos, anneal to form double strands, and then ligate to the linearized vector provided in this kit for CRISPR/Cas9-based gene editing of interest. After transfection, positive mammalian cells expressing Cas9 and gRNA can be screened by Puro resistance, where gene editing usually occurs.