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Cleavage of Lambda DNA with Eco RI Restriction Enzyme
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.
| Cat. No.: | 212 |
| Group Size: | For 10 restriction digestions and 5 gels |
| Time Required: | Complete in 90 minutes |
| Kit Includes: | Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK® Enzyme Grade Water, Eco RI Dryzymes™ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template. |
| All You Need: | DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, White Light Box, & Microwave or Hot Plate. |
| Storage: | Some Components Require Freezer Storage Upon Receipt |
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