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Drosophila Genotyping Using PCR
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
| Cat. No.: | 337 |
| Group Size: | For 10 lab groups |
| Time Required: | Extraction – 50 minutes
PCR Setup – 10 minutes PCR – 2 hours Electrophoresis – 60 minutes Staining – 5 minutes to overnight |
| Kit Includes: | Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, EdvoQuick™ DNA ladder, Drosophila LyphoPrimer™, Drosophila WT LyphoTemplate™, potassium acetate, DNA extraction buffer, Proteinase K, Wild-type and White Drosophila, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentrifuge & PCR tubes. |
| All You Need: | DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), Water Bath, Microcentrifuge & Microwave or Hot plate. |
| Storage: | Some Components Require Freezer Storage Upon Receipt |
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