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GFP Transformation Extension: Colony PCR
Colony PCR represents a simple and easy way to determine whether cloning and transformation experiments were successful. In this experiment, students will use colony PCR to analyze bacteria transformed with pFluoroGreen. A single colony will be used as the DNA template for PCR. The resulting PCR sample will then be analyzed using agarose gel electrophoresis. If the bacteria have been transformed successfully, a PCR product representing the GFP gene will be produced. A bacterial housekeeping gene is amplified at the same time as a positive control. The presence of both bands is indicative of a successful transformation experiment.
This kit is intended for use in conjunction with Edvotek Transformation Kits 222, 223 or 303.
| Cat. No.: | 323 |
| Group Size: | For 10 lab groups |
| Time Required: | Set Up – 30 minutes | PCR – 2 hours or overnight | Electrophoresis – 45 minutes |
| Kit Includes: | Instructions, PCR EdvoBeads™, Colony Primer Mix Concentrate, Control DNA Concentrate, Ampicillin, IPTG, EdvoQuick™ DNA Ladder, TE Buffer, BactoBeads™, ReadyPour™ Luria Broth Agar, Petri Dishes, , ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Liquid Stain, Petri Plates, Sterile Loops, Microcentrifuge & PCR Tubes & Wax Beads. |
| All You Need: | DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a Thermal Cycler), White Light Box (optional), Microcentrifuge, & Microwave or Hot plate. |
| Storage: | Some Components Require Refrigerator and Freezer Storage Upon Receipt |
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