Lighting Up Life: Expression of GFP in C. elegans

Lighting Up Life: Expression of GFP in C. elegans

Scientists can directly manipulate an organism’s genome to produce a phenotype using engineered genes called transgenes. In this experiment, students will use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

SKU: EVT-858 Categories: , , Brand:
Cat. No.: 858
Group Size: For 10 lab groups
Time Required: Growing Bacteria – Overnight

Plating Worms – 15 minutes

Worm Growth – 3-4 Days

Set Up – 30 minutes

PCR – 2 hours or overnight

Electrophoresis – 90 minutes
Kit Includes: Instructions, C. elegans, E. coli OP50 cells, PCR EdvoBeads™ PLUS, GFP primer mix concentrate, EdvoQuick™ DNA ladder, control DNA concentrate, rehydration buffer, sterile water, TE buffer, extraction buffer, ReadyPour™ NGM agar, NGM salts, petri dishes, pipets, tubes, UltraSpec-Agarose™, electrophoresis buffer (50x), 10x gel loading solution, SYBR® Safe DNA stain, FlashBlue™ liquid stain, sterile loops, & wax beads
All You Need: Covered box, fluorescent and dissecting microscopes, timers, water bath, incubator (optional), thermal cycler or three water baths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or UV photodocumentation system, automatic micropipettes with tips, microwave or hot plate, pipet pump, permanent markers, flasks or beakers, hot gloves, distilled or deionized water, sterile water, ice buckets and ice, bleach solution or laboratory disinfectant
Storage: Some Components Require Refrigerator & Freezer Storage

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