Mapping of Restriction Sites on Plasmid DNA

Plasmid and lambda DNA are pre-digested with restriction enzymes – endonucleases that recognize and cut double-stranded DNA within or DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

 

Cat. No.: 105
Group Size: For 8 gels
Time Required: Complete in 45 minutes
Kit Includes: Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets.
All You Need: DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.