PCR Amplification of DNA

In this easy PCR experiment, students will make billions of copies of a small amount of DNA in just 90 minutes! They will just need to mix template DNA & primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students will see the increasing amounts of DNA for themselves, taking samples every few cycles and analyzing them on a DNA gel.

SKU: EVT-330 Categories: , , Brand:
Cat. No.: 330
Group Size: For 10 lab groups
Time Required: PCR Setup – 10 minutes

PCR – 90 minutes

Electrophoresis – 30 minutes

Staining – 5 minutes to overnight

Kit Includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, DNA Standard Marker, Ultra-pure Water, LyphoTemplate™, TE Buffer, UltraSpec-Agarose™, 50X Electrophoresis Buffer, FlashBlue™ Stain, SYBR® Safe DNA Stain, Microcentrifuge & PCR Tubes.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box, (3) Water Baths (if performing experiment without a Thermal Cycler), Microcentrifuge & Microwave or Hot plate.
Storage: Some Components Require Freezer Storage Upon Receipt