Purification of the Restriction Enzyme Eco RI

Purification of the Restriction Enzyme Eco RI

In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses.

SKU: EVT-302 Categories: , , Brand:
Cat. No.: 302
Group Size: For 5 purifications
Time Required: Packing Column – 45 minutes

Restriction Analysis A – 35 minutes

Restriction Analysis B – 50 minutes

Gel Prep – 30 minutes

Electrophoresis – 30 minutes

Staining & Destaining – 2 minutes
Kit Includes: Instructions, E. coli RY (Eco Extract (lyophilized), DEAE-Cellulose, 10x equilibration buffer, 50% glycerol, KCl, Eco RI reaction buffer, qualified water, Lambda DNA, Lambda/Eco RI marker, Eco RI dilution buffer, UltraSpec-Agarose™ powder, concentrated electrophoresis buffer, 10x gel loading solution, chromatography columns, SYBR® Safe stain, 5ml snap-top microcentrifuge tubes, 1.5 mL snap-top microcentrifuge tubes, & 50mL conical tube.
All You Need: Horizontal gel electrophoresis apparatus, power supply, automatic micropipette with tips, balance, water bath, ring stand and clamps, 13 x 100 mm glass test tubes, assorted laboratory glassware, permanent markers and tape, 1.5 mL microtest tubes, microwave, hot plate or burner, pipette pump, 250 mL flasks or beakers, hot gloves, safety goggles and disposable laboratory gloves, distilled or deionized water, LED transilluminator.
Storage: Some Components Require Freezer Storage

Related products