Quick PCR

In this experiment, students will gain an understanding of the traditional three-step Polymerase Chain Reaction (PCR). Using PCR and Agarose Gel Electrophoresis, they will analyze a small section of Lambda DNA in a time-saving two-step process.

 

 

 

SKU: EVT-372 Categories: , , Brand:
Cat. No.: 372
Group Size: For 10 lab groups
Time Required: PCR Setup – 10 minutes

PCR – 30 minutes

Electrophoresis – 30 minutes

Staining – 5 minutes to overnight

Kit Includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoTemplate™ Lambda DNA, TE Buffer, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (2) Water Baths (if performing experiment with a Thermal Cycler), White Light Box (optional), Microcentrifuge & Microwave or Hot plate.
Storage: Some Components Require Freezer Storage Upon Receipt