VNTR Human DNA Typing Using PCR

VNTR Human DNA Typing Using PCR

In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. In this kit, students will type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.

SKU: EVT-334 Categories: , , Brand:
Cat. No.: 334
Group Size: For 25 students
Time Required: Extraction – 30 minutes

PCR Setup – 10 minutes

PCR – 90 minutes

Electrophoresis – 30 minutes

Staining – 5 minutes
Kit Includes: Instructions, PCR EdvoBeads™, LyphoPrimer™ mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (2) Water Baths, Microcentrifuge, Microwave or Hot plate.
Storage: Some Components Require Freezer Storage Upon Receipt

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