Water Quality Testing III: Multiplex PCR Testing of Water Contaminants

Water Quality Testing III: Multiplex PCR Testing of Water Contaminants

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

SKU: EVT-953 Categories: , , Brand:
Cat. No.: 953
Group Size: For 25 students
Time Required: Extraction – 60 minutes

PCR Setup – 10 minutes

PCR – 2 hours

Electrophoresis – 60 minutes

Staining – 5 mins or overnight
Kit Includes: Instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.
Storage: Some Components Require Refrigerator or Freezer Storage Upon Receipt

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